Figure 1. Scototaxis arena and ethanol administration schedule used with control, weekly-binge, and daily-moderate groups.

(A) The scototaxis arena measured 9.5 cm wide, 9.5 cm deep, and 55 cm long, with black walls covering one half of the arena, white walls covering the other half, and white flooring throughout; water depth was maintained at 5 cm. (B) Ethanol administration and testing took place over a 30 day period. The daily-moderate group (•) received ethanol (0.2%) each day for 21 days of the exposure period, whereas the weekly-binge group (○) received ethanol (1.4%) only on the 7th, 14th and 21st days. All groups, including control, underwent the same daily procedures with the exception of ethanol administration. All animals were tested (T) on days 23 (2^nd day of withdrawal) and 30 (9^th day of withdrawal).