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. Author manuscript; available in PMC: 2013 May 13.
Published in final edited form as: J Cell Physiol. 2011 Aug;226(8):2052–2062. doi: 10.1002/jcp.22543

Figure 2. Effect of OxPAPC on interaction between adherens junctions and tight junctions.

Figure 2

Figure 2

A: Cells were stimulated with OxPAPC (10 μg/ml) for various time periods or left untreated followed by immunoprecipitation of p120-catenin. VE-cadherin and β-catenin content in the immunoprecipitates was detected with specific antibodies. Equal protein loading was confirmed by re-probing of membranes with p120-catenin antibody. B and C: HPAEC were stimulated with OxPAPC (10 μg/ml, 30 min). Co-immunoprecipitation using antibody to VE-caherin (B) or ZO-1 (C) was performed, and associated proteins were detected by western blot with corresponding antibodies. Result of densitometry shown as mean ± SD, * p<0.05 D: EC monolayers grown on glass coverslips were stimulated with OxPAPC (10 μg/ml, 30 min) followed by double immunofluorescence staining for p120-catein (red) and ZO-1 (green). Merged images depict areas of protein co-localization which appear in yellow and marked by arrows. Shown are representative results of three independent experiments.