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. 2013 Apr 24;97(6):1235–1242. doi: 10.3945/ajcn.112.057182

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FIGURE 2. — Weighted relative frequency for serum 25-hydroxyvitamin D of National Adult Nutrition Survey samples (n = 1118) measured by using the Immuno Diagnostic Systems Ltd enzyme-linked immunosorbent assay (dotted green line) and standardized liquid chromatography–tandem mass spectrometry at University College Cork (dashed red line) and predicted as per Vitamin D Standardization Program protocol 2 [the black line with shaded area represents 95% confidence bands as estimated by using the method of Fiorio (25)].