Fig. 1.

RT-PCR of CRF₁ in the human gastrointestinal tract from four male healthy subjects. A. Gel images of PCR products. PCR products were separated by 1% agarose gel electrophoresis and visualized with ethidium bromide. Glyceraldehyde-3-phosphate dehydrogenase ⁽GAPDH) was used as an internal control to assure cDNA quality and equal loading. E: esophagus, S: stomach (fundus), P: stomach pylorus, D: duodenum, J: Jejunum, I: ileum, Ce: cecum, aC: ascending colon, tC: transverse colon, dC: descending colon, sC: sigmoid colon, R: rectum. B. Quantitative analysis of PCR products. The densitometric analysis of PCR products was performed by using NIH Image system (Scion Corporation, Frederick, MD) and standardized by taking the ratio to that of GAPDH in each sample respectively. * p<0.05 vs ileum (I). n=4.