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. 2013 May 14;7:91. doi: 10.3389/fncir.2013.00091

Figure 2.

Figure 2

Cx35 distribution in the CNS of 2 dpf larvae. Larvae are dorso-ventrally placed with anterior on top in all panels. All panels except (F) show single optical sections taken at a z-resolution of 0.44 μm. (A) Ventral telencephalon showing the anterior commissure (AC), supra-optic tract (SOT), and the post-optic commissure (POC). (B) Dorsal view of forebrain showing Cx35 positive cells in the dorsal pallium (Pd). The posterior commissure can be seen (arrowhead) while the habenulae (Hab) and the pineal body (P) lack Cx35 immunoreactivity. (C) Dorsal view of the optic tectum (OT). A single optical section showing Cx35 immunoreactivity in the developing neuropil region (arrowheads). (D) Cell bodies (arrowheads) in hindbrain lining the rhombencephalic ventricle. (E) Ventral hindbrain (Hb) showing intense Cx35 immunoreactivity in fibers coursing through it. (F) Zoomed image of a Mauthner neuron (M, yellow outline) showing punctate Cx35 immunoreactivity. The image is a maximum intensity projection of optical slices collected from a 16 μm thick tissue slice encompassing the Mauthner neuron. Scale bar for all panels: 20 μm.