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. 2013 May 14;7:91. doi: 10.3389/fncir.2013.00091

Figure 3.

Figure 3

Distribution of Cx35 immunoreactivity in 3 dpf larvae. All panels except (C) show dorso-ventrally placed larvae, with the anterior end of the animal at the top of the panel. All panels are single optical sections taken at a z-resolution of 0.44 μm. (A) Ventral forebrain showing immunoreactivity in the anterior commissure (AC) and in the tract of the post-optic commissure (TPOC). (B) Dorsal telencephalon and rostral mesencephalon. Staining in cells of the dorsal pallium (Pd) and in the posterior commissure (PC) can be seen. (C) Lateral view of forebrain. Dorsal is on top and anterior to the left. Cx35 immunoreactivity is seen in the anterior commissure, in the supra-optic tract and in thick fiber tracts in ventral diencephalon. Numerous cell bodies can also be seen in the vicinity of the anterior commissure. (D) Intense Cx35 immunoreactivity in the optic tectal (OT) neuropil. (E) Ventral hindbrain (Hb) showing fiber tracts criss-crossing the hindbrain. (F) Zoomed image of a single Mauthner neuron (M) showing intense Cx35 immunoreactive puncta. The approximate boundary of the Mauthner neuron as seen in DIC is shown in yellow. Scale bar for all panels: 20 μm.