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. 2013 May 14;7:91. doi: 10.3389/fncir.2013.00091

Figure 4.

Figure 4

Cx35 immunostaining in 4 dpf larvae. Larvae are dorso-ventrally placed with anterior on top in all panels. All panels show single optical sections taken at a z-resolution of 0.44 μm. (A) Dorsal forebrain showing Cx35 staining in the paired habenulae (Hab) and in the habenular commissure (Chab). Cell bodies in dorsal pallium (Pd) continue to stain positive. No staining is seen in the pineal body (P). (B) Ventral telencephalon showing Cx35 immunoreactivity in the olfactory bulb (OB), anterior commissure (AC), supra-optic tract (SOT), post-optic commissure (POC), and the tract of the post-optic commissure (TPOC). (C) Cx35 immunoreactivity in fiber tracts in the ventral diencephalon (Di). (D) Intense Cx35 immunoreactivity in the optic tectal neuropil (OT) and in the intertectal commissures (Ctec; arrowhead) in the mesencephalon. Tectal cell bodies are also weakly labeled. (E) Weak staining is seen in the cerebellum within the molecular layer (ML) and in the corpus cerebelli (CCe). (F) Zoomed image of a single Mauthner neuron (M) showing intense Cx35 immunoreactive puncta. The approximate boundary of the Mauthner neuron as seen in DIC is shown in yellow. Scale bar for all panels: 20 μm.