Figure 5.

Distribution of Cx35 immunoreactivity in the CNS of 6 dpf larvae. Larvae are dorso-ventrally placed with anterior on top in all panels. All panels show single optical sections taken at a z-resolution of 0.44 μm. (A) Ventral telencephalon showing staining in anterior commissure (AC) and the olfactory bulb (OB). A few faintly stained cell bodies can be seen in the vicinity of the anterior commissure. (B) Dorsal forebrain showing Cx35 staining in the habenulae (Hab), dorsal pallium (Pd), and the posterior commissure (PC; arrowhead). (C) Magnified dorsal view of the optic tectum (OT) showing intense staining in the neuropil area. Faint labeling in cell bodies is also seen (arrowhead). (D) Cx35 staining in the mesencephalon showing intense staining in the optic tectal lobes in the neuropil area as well as in intertectal commissures (Ctec; arrowhead). The corpus cerebelli (CCe) can also be seen. (E) Cerebellar staining showing punctate pattern within the corpus cerebelli (CCe) and the molecular layer (ML). (F) Strong staining present in radial glia (RG) lining the rhombencephalic ventricle. Arrowhead marks the process from one glial cell coursing laterally. (G) Ladder-like staining pattern in ventral hindbrain (Hb). Scale bar for all panels: 20 μm.