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. 2013 May 14;7:91. doi: 10.3389/fncir.2013.00091

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Copyright © 2013 Jabeen and Thirumalai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

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Cx35 staining pattern in the CNS of 15 dpf zebrafish. Larvae are dorso-ventrally placed with anterior on top in all panels. All panels show single optical sections taken at a z-resolution of 0.44 μm. (A) Ventral telencephalon showing punctate Cx35 immunoreactivity in the anterior commissure (AC) and the olfactory bulb (OB). (B) Telencephalon 50 μm from the ventral surface showing staining in the anterior commissure (AC) and in the olfactory bulb (OB). The telencephalic ventricle (TelV) can be seen. (C) Mesencephalon, dorsal view showing staining in the optic tectal (OT) neuropil and in intertectal commissures (Ctec, arrowhead). (D) Habenulae (Hab) and the habenular commissure (Chab; arrowhead). Polygonal cell bodies can be seen in the habenulae (E) Cerebellum showing intense Cx35 immunoreactivity in the corpus cerebelli (CCe) and the molecular layer (ML) (F) Ventral Hindbrain (Hb) showing a ladder-like pattern that includes Cx35 staining in the lateral longitudinal fasciculus (LLF) and hindbrain commissures. The rhombencephalic ventricle (RV) can be seen. (G) Intense punctate Cx35 immunoreactivity in the Mauthner neurons (M; arrowheads). The approximate boundaries of the Mauthner neurons is drawn in yellow. Scale bar for all panels: 20 μm.