Figure 3.
Segmental organization of combinatorial patterns of gene expression within rostral medulla hindbrain lineages. Pseudo-color fluorescent confocal mosaic hemi-sections from P0 transgenic mice at the level of pseudo-rhombomere 7 (A–F) and 8 (G–L). Images from Lmx1b-βgal (A–C,G–I), Dbx1-βgal (D,E,J,K), and Atoh1-Cre; R26YFP (F,L) mice showing localization of lineage reporter (green) β-Gal (A–E,G–K) or YFP (F,L) with reference genes (magenta) TH (A,G) FoxP2 (B,F,H,K,L) Phox2b (C,I) Pax2 (D), SST2aR (E), or SST (J). Boxed region in each image is expanded to right showing overlapping (top), and single colors magenta (middle), and green (bottom). Arrows indicate co-expression. Arrowheads indicate absence of co-expression. See Figures 2C,E for identification of specific regions. Scale bar = 500 μm.