Figure 7.

Pseudo-rhombomere 9 specific patterns of gene expression. Pseudo-color fluorescent confocal mosaic images from P0 transgenic mouse at the level of pseudo-rhombomere 9. (A) Schematic image modified from Figure 2G indicating location of co-expressing populations shown in (B–I). (B–I) Images from Dbx1-βgal (B,D,E), Lmx1b-βgal (C), wild type (F), and Atoh1-Cre; R26YFP (G–I) mice showing localization of lineage reporter (green) β-gal (B–E) or YFP (G–I) with SST2aR (B,C,H), NK1R (D,E,G), or Lhx9 (I). Text color indicates gene color in images. Boxed region in (B–E,G–I) is expanded to right showing overlapping (right, top), and single colors magenta (middle), and green (bottom). Boxed region in (E) is expanded (bottom left) with single colors red (right, top), green (middle), and blue (bottom). Scale bar = 500 μm.