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. 2013 May 14;3:122. doi: 10.3389/fonc.2013.00122

Figure 2.

Figure 2

Identification of relevant conformational epitopes on the HER2 oncoprotein by LFPD. LFPD structure (A). The EC domain of rat HER2 was divided into 11 fragments of 106 amino acids: 6 contiguous core fragments + 5 fragments that overlap the previous ones. Screening of sera from mice vaccinated with EC-TM plasmid by an ELISA assay based on LFPD (B). Both sera from BALB/c (blue line) and BALB-neuT (red line) mice recognized rat1 (1–106 aa), rat2 (107–216 aa), rat9 (267–376 aa), and rat11 (487–596 aa) conformational epitopes, although tolerant mice sera showed lower reactivity. Sera from mice vaccinated with a truncated form of HER2 (EC4-TM) recognized rat6 (547–656 aa), rat9, and rat11 fragments (orange line). Pepito based analysis of rat HER2 (C). Red zones indicate the maximum probability of finding a conformational epitope. Adapted from Gabrielli et al. (2013).