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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Microb Pathog. 2012 Aug 29;53(0):234–242. doi: 10.1016/j.micpath.2012.08.005

Fig. 4.

Fig. 4

Effect of cholesterol depletion on the bacterial invasion assessed by CSLM. Ca9-22 cells were incubated with DMSO (control) or 10 mM methyl-beta-cyclodextrin (MβCD) or for 30 min at 37 °C, prior to infection. (a) P. gingivalis 33277 control, (b) P. gingivalis + MβCD, (c) F. nucleatum TDC 100 control, (d) F. nucleatum TDC 100 + MβCD, (e) P. gingivalis + F. nucleatum control, (f) P. gingivalis + F. nucleatum + MβCD. Bar = 10 μm. Internalized bacteria were stained red, while extracellular bacteria were shown green-yellow. For dual-infection, anti-P. gingivalis and anti-F. nucleatum antisera were used. The host cell cytoskeleton stained with phalloidin appeared blue. (For interpretation of color referred in this figure legend, the reader is referred to web version of the article.)