Table 1.

Effect of MβCD on bacterial invasion of Ca9-22.

Infection	Invasion of Ca9-22 (% of untreated control)
Mono-infection
P. gingivalis	33.4 ± 18.4*
F. nucleatum	13.3 ± 14.5*
Dual-infection
P. gingivalis	14.4 ± 11.4*
F. nucleatum	27.2 ± 15.9*

Ca9-22 cells were infected by P. gingivalis 33277 and/or F. nucleatum TDC 100 for 2 h in vitro. An antibiotic protection assay was used to quantify intracellular bacteria. In order to disrupt lipid rafts, cells were pretreated for 30 min with 10 mM MβCD. Values given as means ± standard deviations of triplicate independent determinations from a typical experiment.

^*Statistically significantly different from untreated control (p < 0.001) by ANOVA with Bonferroni multiple comparisons test.