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. 2013 May 13;201(4):531–539. doi: 10.1083/jcb.201211160

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© 2013 Takáts et al.

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Figure 3. — Syx17 binds to usnp and VAMP7. (A) Coimmunoprecipitation shows that Syx17-FLAG binds to both HA-usnp and HA-VAMP7 in cultured Drosophila cells. Note that usnp facilitates the interaction of overexpressed VAMP7 with Syx17 (both lacking transmembrane domains). (B) Endogenous usnp coimmunoprecipitates with endogenous Syx17. (C) Endogenous Syx17 coimmunoprecipitates with endogenous usnp. gp, guinea pig; IP, immunoprecipitation; IB, immunoblotting.