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. 2013 May 13;201(4):531–539. doi: 10.1083/jcb.201211160

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© 2013 Takáts et al.

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Figure 4. — Syx17 is recruited to completed autophagosomes. (A and B) Endogenous Syx17 colocalizes with both endogenous (A) and GFP-tagged (B) Atg8a in starved fat body cells. (C and D) No colocalization is observed between Syx17 and the phagophore marker Atg5 (C) or late endosomes and lysosomes, labeled by GFP-dLAMP (D). (E) Syx17 and Atg8a do not colocalize in Atg2 mutants that accumulate Atg8a-positive stalled phagophores. Insets show merged images (top), Syx17 channels (middle), and relevant green channels (bottom) enlarged from boxed areas in A–E. (F) Immunogold labeling reveals that Syx17 is associated with the outer membrane of autophagosomes (AP). Bars: (A and C–E) 20 µm; (B) 20 µm; (F) 100 nm.