Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 May 13;201(4):613–629. doi: 10.1083/jcb.201206006

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Davalos et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 8. — ATM is dispensable for HMGB1 secretion. (A) PRE or SEN (XRA) HCA2 cells and two A-T derived fibroblast strains (AT2SF, AT5283) were immunostained for HMGB1 (red). Nuclei were stained with DAPI (blue). Bars,10 µM. (B) Cells in A were scored for SA-β-Gal and BrdU incorporation (24-h pulse). Error bars = SEM of two experiments. (C) CM from cells in A were assessed for HMGB1 secretion by ELISA. Error bars = SEM of two experiments. (D) Lysates from HCA2 cells infected with lentiviruses carrying GFP (Control) or ATM (KD) shRNA and lysates from A-T fibroblasts (AT2SF) were analyzed for HMGB1 and actin by Western blotting. HMGB1 signals were normalized to actin, and are shown relative to shGFP.