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Effect of glutamate in P7 hESC NPs. A representative trace (A) showing the [Ca2+]i responses after the application of different concentrations of glutamate (1–100 μM). A bar diagram (B) showing the amplitude (mean±SEM) of the [Ca2+]i responses to the application of increased concentrations of glutamate. The number of cells tested is given in the parentheses. ***P=0.0005.
