Table 2.
The Number of Cells Responding to Various Physiological Stimuli in Different Passages of Human Embryonic Stem Cell-Derived Neural Precursors (P6–P10 NPs)
Number of cells tested | P6 NPs n=44 | P7 NPs n=44 | P8 NPs n=45 | P9 NPs n=45 | P10 NPs n=44 |
---|---|---|---|---|---|
Cells sensitive to 1 or more of applied stimuli | 41%±1.6% | 59%±2.5% | 49%±1.1% | 15%±1.7% | 7%±0.2% |
K+ 50 mM | 11%±1% | 50%±3.9% | 32%±4% | 4%±2.2% | 2.3% |
ATP 100 μM | 7%±1% | 46%±2.2% | 24%±3.5% | 9%±1.9% | 5%±2.3% |
Glutamate 50 μM | 27%±1.3% | 57%±0.3% | 9%±1.3% | 4%±2.2% | 0 |
GABA 10 μM | 7%±1% | 5%±2.3% | 16%±1.1% | 0 | 0 |
Caffeine 20 mM | 4%±2% | 18%±2.1% | 16%±1.1% | 4%±2.1% | 2.3% |
ATP, adenosine-5′-triphosphate.