Table 3.

Expression of Various Neural Markers and Ca²⁺ Sensitive Channels in P6–P10 hESC NPs

	P6 NPs	P7 NPs	P8 NPs	P9 NPs	P10 NPs
Neural markers
Nestin	++	++	++	++	++
NeuN	++	++	++	+	+
βIII tubulin	+++	+++	+++	++	++
MAP2	−	−	−	−	−
Glutamate	+	+++	−	−	−
GABA	+	−	+	−	−
GFAP	++	+++	++	+	−
S100	+	++	++	++	++
OLIG	−	++	−	−	−
GS	−	++	−	−	−
NG2	−	−	−	−	+
Ca2+-sensitive channels
L-type VOCC	++	+++	++	++	++
N-type VOCC	−	−	−	−	−
P/Q-type VOCC	++	+++	++	++	++
RyR 1	+++	+++	+++	+++	+++
RyR 2	+	+	+	+	+
RyR 3	++	+++	++	++	++

“−”-negative immunostaining.

“+”-faint positive immunostaining/few cells express the marker.

“++”-positive immunostaining/many cells express the marker.

“+++”-very positive/majority of cells express the marker.

VOCC, voltage-operated Ca²⁺ channels.