FIG. 2.

Cell membrane capacitances, resting membrane potentials (RMPs), and action potential (AP) types in the differentiating neurons. (A) Whole-cell patch clamp recording of hiPS cell-derived neurons (upper panel) revealed a continuously increasing cell size (middle panel) and the development of cell RMPs from a depolarized to hyperpolarized level (bottom panel). (B) The same experiment and similar observations in human embryonic stem (ES) cell-derived neurons. (C) Abortive, single, and multiple, repetitive APs obtained in differentiating neurons derived from hiPS and ES cells. *P<0.05, comparing the correlated groups as indicated. n=6–21 in each group.