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. 2012 Dec 23;22(10):1551–1563. doi: 10.1089/scd.2012.0556

FIG. 3.

FIG. 3.

Voltage-gated Na+ currents (INa) in the differentiating neurons. (A) The pure INa was evoked by voltage steps from −60 to +60 mV in the presence of Cs+, tetraethylammonium (TEA), and Cd2+. INa was suppressed by its specific blocker tetrodotoxin (TTX, 1 μM). Cell membrane potential was held at −70 mV. (B) The current and voltage (I/V) relationship of INa in the absence and presence of 1 μM TTX. (C) In hiPS cell-derived neurons, the peak amplitude of INa was small at week 1, and markedly increased at week 2, but did not change after week 2. (D) The peak amplitude of INa was small in neurons firing abortive APs, and dramatically increased in neurons firing single APs, but did not show any difference among those neurons firing single, multiple, and repetitive APs. (E, F) The developmental changes of INa in human ES cell-derived neurons were similar to those observed in hiPS cell-derived neurons. *P<0.05, comparing the correlated groups as indicated. n=6–12 in each group.