FIG. 1.

Dopamine (DA) receptor mRNA levels during the dibutyryl cAMP (dbcAMP)-based differentiation protocol. (A) Time course of the differentiation protocol indicating media additives during each stage (Stages 1–5). (B₁) rtPCR of tyrosine hydroxylase (TH), 5 DA receptors, and the housekeeping gene ACTB. CP, cortical plate; SVZ, subventricular zone; Cx, cortex with all zones included; GE, ganglionic eminence; ES, stage 1 undifferentiated H9 human embryonic stem cell (hESC) colonies; EB, stage 2 embryoid bodies; NE, stage 3 neuroepithelia; Exp, stage 4 expansion of neuroepithelia; Diff., stage 5 final neurodifferentiation. (B₂) Quantitation of hESC band intensities relative to Stage 1 (ES). rtPCR, reverse transcriptase PCR.