FIG. 10.

Effect of DA on H9 differentiation in PA6 coculture. H9 cells were seeded on PA6 feeder layers and exposed to the indicated concentrations of DA continuously. Cells were fixed on day 14, and dual immunofluorescent staining for TH and TUJ1 was performed. (A1, A2) 0 DA: TH, TUJ1 staining. (B1, B2) 100 μM DA: TH and TUJ1 staining. Scale bar=200 μm. (C) The 3 brightest TH fields were photographed per coverslip. Signal intensities were quantitated. Ratio of total TH signal/TUJ1 signal was calculated. **Kruskal–Wallis one-way analysis of variance on Ranks P=0.002. (D) Number of TH⁺ clusters of cells per coverslip. n=4–5 coverslips per group. Error bars=SEM.