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. 2013 Jan 3;22(10):1522–1540. doi: 10.1089/scd.2012.0150

FIG. 4.

FIG. 4.

Effect of dopaminergic drugs on creation of EBs and neural progenitors. (A) Time course of the experiment. Normal EBs (Control) and irregular EBs treated with the indicated drugs. (B) Normal EBs (left) and irregular EBs treated with 0.5 μM SKF83566 (right). (C) Cultures were exposed to the indicated drugs from days 3 to 8 of our standard differentiation protocol. EBs were scored for normal smooth surface versus irregular surface. 1 and 2 are independent experiments. n=2–3 wells per treatment. Error bars=SEM. Experiment 1: Kruskal–Wallis one-way ANOVA on ranks, P=0.018. For SKF83566 versus control P<0.05 using Dunn's pairwise comparison. Experiment 2: Kruskal–Wallis one-way ANOVA on ranks, P=0.007, for SKF83566 versus control P<0.05 using Dunn's pairwise comparison. The EBs in (B and C) were seeded to create the colonies in (D) and (E). (D) Normal neuroepithelial colony (left) and non-neuroepithelial colony after treatment with 0.5 μM SKF83566 (right). (E) Neuroepithelial stage cultures were scored for % neuroepithelial colonies on day 8. For Experiment 1, Kruskal–Wallis one-way ANOVA on ranks P=0.011; Control versus SKF83566 Mann–Whitney Rank sum test P=0.029. For Experiment 2, ANOVA P<0.001; Tukey test on Control versus SKF83566 P<0.001. All scale bars=500 μm.