FIG. 5.

Effect of DA receptor ligands on neurites. (A) Time course of the experiment. Cultures were treated as indicated from days −3 to 8 (early treatment) or from days 14 to 28 (late treatment) of our standard differentiation protocol. All cultures were maintained until day 36 and neurites quantitated. (B) Phase-contrast image (inverted) showing neurites in the control well. (C) Neurites in wells treated with DA [10 μM]. (D) Quantitation of neurites exposed to “early treatment.” Scale bar=500 μm. (E) Quantitation of the “late treatment” data. n=9 fields counted per treatment. One-way ANOVA, P=0.038. Tukey test for Control versus DA late treatments P=0.045.