Fig. 3.

Effect of rHDL on phosphorylation of AMPK, ACC and Akt in L6 myotubes. Myotubes were serum starved for 4 h then treated for 60 min with rHDL (14 µmol/l apoA-I in rHDL), 2 mmol/l or 0.4 mmol/l phenformin, 100 nmol/l or 1 nmol/l insulin, or control (C). Densitometric analysis of lysates subjected to western blot analysis using antibodies for (A) phospho-AMPK and AMPK, (C) phospho-ACC and AMPK, and (E) phospho-Akt and Akt. (B, D, F) Representative western blots. n = 3, ± SEM; *P ≤ 0.05, **P ≤ 0.01.