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. 2013 May;54(5):1410–1420. doi: 10.1194/jlr.M035774

Fig. 2.

Fig. 2.

The ubiquitination of IDOL and the LDLR does not exclusively depend on K48- or K63-linked ubiquitin in hepatocyte cell lines. A: Hep3B and HepG2 cells were cotransfected with FLAG-tagged IDOL and HA-tagged wild-type K48R or K63R ubiquitin. Cells were treated with 25 μM MG132 for 4 h before lysis in RIPA buffer. Proteins in the lysates were analyzed by immunoprecipitation and immunoblotting. B: Hep3B and HepG2 cells were cotransfected with FLAG-tagged IDOL, V5-tagged LDLR, and HA-tagged wild-type K48R or K63R ubiquitin. Cells were treated with 50 nM bafilomycin (Bafil) for 4 h before lysis in RIPA buffer. Proteins in the lysates were analyzed by immunoprecipitation and immunoblotting. IP, immunoprecipitation; WB, Western blotting.