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LDLR degradation induced by LXR activation does not require K48- or K63-specific ubiquitin linkage. U2OS cell lines were cultured in the absence (−) or presence (+) of 1 μg/ml tetracycline (Tet) for 108 h. In the absence or presence of tetracycline, cells were treated with 5 µM simvastatin and 100 µM mevalonic acid, with or without 1µM GW3965 for 12 h. Cells were lysed in RIPA buffer and proteins in the lysates were immunoblotted with the indicated antibodies.
