Skip to main content
. 2013 May;54(5):1410–1420. doi: 10.1194/jlr.M035774

Fig. 8.

Fig. 8.

Inhibition of UBE2N/V1 expression and function does not prevent LDLR degradation. A: Quantitative PCR analysis of RNA samples collected from HEK293T cells tranfected with the indicated siRNAs. B: HEK293T cells were cotransfected with LDLR and IDOL, together with the indicated siRNAs. Cells were lysed in RIPA buffer and proteins in the lysates were immunoblotted with the indicated antibody. C: Immunoblot analysis of protein levels in HEK293T cells transfected with WT or dominant negative (DN) UBE2D2 or UBE2N, in addition to LDLR and IDOL.