Fig. 3.

2OHOA induces de novo ceramide synthesis. U118 cells exposed to 2OHOA for 6 h (A) and 24 h (B) were pulse-labeled with [³H]palmitic acid for 5 min before the radioactive lipids were separated by TLC and quantified by liquid scintillation counting. HexCer includes GluCer+GalCer, [³H]SP and [³H]sphingolipids. Values represent the mean ± SEM (n = 3). Asterisks (*) indicate a significant effect of the treatment compared with controls: *P < 0.05, **P < 0.01, and ***P < 0.001.