Fig. 6.

The RNA-binding activity of Dnd1 is required for its function during vegetal cortical microtubule assembly in activated eggs and dorsal development in fertilized eggs. (A) Confocal images showing the formation of vegetal cortical microtubules in uninjected control (upper/left), AS-oligo-injected (upper/right), AS-oligo- and dnd1-injected (lower/left), and AS-oligo- and Y125C-injected (lower/right) eggs (40 minutes post-needle-pricking). Fixed eggs were bisected and the vegetal halves were stained with an anti-Tubulin antibody. (B) RT-PCR showing that overexpression of the wild-type dnd1, but not Y125C, rescued the expression of noggin, chordin, Xnr3 and siamois in Dnd1 knockdown embryos (stage 10.5).