Figure 1. ORM2 confers specific resistance to iron toxicity in a variety of physiological contexts.
A. Strains were grown in SD medium lacking uracil (-URA) supplemented with the indicated metals. These, and all subsequent assays show 10-fold dilution series from left to right. For the bottom panel, plates were incubated at 30°C. B. Cells expressing Orm1p or Orm2p HA-tagged at their chromosomal loci (Chr), transformed with either the YEp24 vector, or with plasmids containing HA-tagged Orm1p or Orm2p (pORM1/2-HA) were grown in 7mM FeCl3, and the levels of Orm1/2p analyzed by immunoblot. Upper panels are two different exposures to detect endogenous and overexpressed Orm1/2p. C. Strains were grown on ethanol-glycerol carbon source lacking uracil (URAEG), without or with 5mM FeCl3. D. Legends as in A.