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. 2013 Apr;48(4):503–510. doi: 10.1165/rcmb.2012-0150OC

Figure 2.

Figure 2.

Cytokine concentration dependencies of eosinophil adhesion to PN. (A) Adhesion to full-length PN coated at 5 μg/ml followed by postcoating with FBS of purified blood eosinophils in the absence or presence of different concentrations of IL-5 (squares), IL-3 (triangles), or granulocyte/macrophage colony–stimulating factor (GM-CSF) (circles). Data shown are means (±SEM) (n = 3 donors). ANOVA for curves of: IL-5, P = 0.005; IL-3, P = 0.02; and GM-CSF, P = 0.02. Post test: †P ≤ 0.05 versus no cytokine. (B) Adhesion of purified blood eosinophils in the absence or presence of 10 ng/ml IL-5, IL-4, or IL-13 to PN lacking exons 17, 18, 19, and 21 (PN0) coated at 10 μg/ml followed by postcoating with FBS or FBS alone. Data shown are means (±SEM) (n = 3 donors). †P ≤ 0.05 versus no cytokine on PN0; *P ≤ 0.05 versus FBS (t test).