Figure 5.

Increased CD4⁺ T cells in lungs of mice prone to BALT-like infiltrates and increased major histocompatibility complex (MHC) Class II expression on B cells. The right upper and middle lobes of lungs from H-L-g7xK, K/BxN, and wild-type (WT) control mice (BxN or B6-Ag7) were digested and evaluated by flow cytometry. The remaining lung tissue was reserved for histology. (A) Percentages and absolute numbers of CD4⁺ T cells (×10³) and CD19⁺ B cells (×10³) in the lungs. (B) Expression of MHC Class II according to mean fluorescence intensity (MFI) was measured on dendritic cells (CD11b⁺CD11c⁺SSClo) and CD19⁺ B cells. (C) The extent of lymphocytic infiltrates was scored by a blinded observer on an arbitrary scale of 1–4. (D) A significant correlation was found between MHC Class II MFI and lymphocytic infiltration score (Pearson correlation, r = 0.8127). *P < 0.5, **P < 0.01, ***P < 0.001.