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. Author manuscript; available in PMC: 2013 May 14.
Published in final edited form as: J Immunol. 2011 Oct 14;187(10):5328–5335. doi: 10.4049/jimmunol.1101445

Figure 5. IL-23 and IL-1β reconstitute the ability of egg-stimulated IL-12p40−/−DCs to induce IL-17 production by TCR Tg CD4 T cells.

Figure 5

DCsfrom WT CBA or CBA IL-12p40−/− mice plus TCR Tg CD4 T cells were cultured in the presence of eggs and recombinant cytokines or IL-1Raat indicated concentrations for 48h. (a) Culture supernatants were assayed for IL-17 by ELISA. (b) Il17, (c) Il22, (d) Cxcl1, (e) Il23r and (f) Rorcexpressionwas determined by real-time quantitative RT-PCR. Each DC population was obtained from two mice; CD4 T cells from 3–5 mice. Cytokine levels are expressed as means of triplicate ELISA determinations ± SD. For RT-PCR data, each bar represents the mean mRNA level from duplicate determinations ± SD. Data are from one representative experiment of two to three.