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. Author manuscript; available in PMC: 2013 May 14.
Published in final edited form as: Mol Immunol. 2011 Aug 6;48(0):2151–2158. doi: 10.1016/j.molimm.2011.07.012

Fig. 1.

Fig. 1

Complement activation in the retina of Lewis rats with increased IOP. (A) IOP of eyes treated with laser (■) and the control eyes not treated with laser (□) measured at different time points using Tonolab tonometer. (B-E) Immunofluorescent staining of C3 slit products (B and C) and MAC (D and E) in retina of rat with normal IOP (B and D) and elevated IOP (C and E) at week 6 post-laser. Objective magnification: 10X. (F) Western blot analysis for C3 split products (upper panel) and MAC (lower panel) in the retina of glaucomatous and control eyes at 6 weeks post laser. For the panel showing C3 split products (upper panel), lane 1 represents the molecular weight marker (Magic Marker XP, Invitrogen, Carlsbad, CA), lane 2 represents the control eyes not treated with laser (normal IOP) and lane 3 represents the eyes with elevated IOP at 6 weeks post-laser. For the lower panel showing MAC, lane 1 represents the control eyes not treated with laser (normal IOP) and lane 2 represents the eyes with elevated IOP at 6 weeks post-laser. Bands with strong and equal intensity of β-actin represent equal protein loading in each well. (G and H) Cumulative data for densitometric analysis of C3 (G) and MAC (H) Western blots from three separate experiments is shown. The intensity of protein bands was quantitated using an image analyzer, and the relative intensity was expressed as the ratio of the intensity of C3 (G) and MAC (H) bands to the intensity of β-actin bands. GCL: ganglion cell layer. *p<0.05.