Table 2.

Primers used in this study

Primer	Sequence a
delete_XmaI_gltK_up_F	5′-CCCCCCGGGCCAAGCAGGTACGATTGGAT-3′
delete_EcoRI_gltK_up_R	5′-AACCGGAAAGCAGAGAATTCTCGAAAACAATGAAACTTCAACA-3′
delete_EcoRI_gltK_dn_F	5′-TCGAGAATTCTCTGCTTTCCGGTTACTTGG-3′
delete_XhoI_gltK_dn_R	5′-CCGCTCGAGGGAAGGATCACACCGATGAC-3′
check_gltK_up	5′-CGGAACGTTAATGGCAATCT-3′
check_gltK_dn	5′-CCGTACCAATCGTACCGATAA-3′
delete_XmaI_gspA_up_F	5′-CCCCCCGGGCCTCCTTTTTGGACTTTCTCG-3′
delete_EcoRI_gspA_up_R	5′-ACCACATTTAGCTGCAGAATTCGACGGCTTTCCGTTGTGTAG-3′
delete_EcoRI_gspA_dn_F	5′-CGAATTCTGCAGCTAAATGTGGTACGAA-3′
delete_XhoI_gspA_dn_R	5′-CCGCTCGAGGCCAAGTGAAAGCTTTGGAA-3′
check_gspA_up	5′-GCTCGAATTCTTCGATTGCT-3′
check_gspA_dn	5′-TGATGAGCCGTTAAATGGAA-3′
complement_BamHI_gltK_F	5′-ACGGGATCCTTTTAGCAATCGTAGCTGGTTT-3′
complement_XhoI_gltK_R	5′-ACCGCTCGAGCGTGAATTTTCAAGTGCTC-3′
complement_BamHI_gspA_F	5′-ACGGGATCCTGAAAAACCTTCGATCGTTCA-3′
complement_XhoI_gspA_R	5′-ACCGCTCGAGTCCATTCCTACTCCCCCTCT-3′
pAT392_EcoRI_lox66_genta_F	5′-AGGGAATTCTACCGTTCGTATAGCATACATTATACGAAGTTATG ATAAACCCAGCGAACCATTTGAGG-3′
pAT392_EcoRI_lox71_genta_R	5′-CTCCGAATTCTACCGTTCGTATAATGTATGCTATACGAAGTTATT CAATCTTTATAAGTCCTTTTATAA-3′

^a Restriction sites are underlined.