Table 1.
Two steps of aqueous two phase partitioning of alkaline protease from farmed giant catfish viscera
| Step | Phase | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Purification (fold) | Recovery (%) |
|---|---|---|---|---|---|---|
| Crude extracta |
- |
2,916.67±11.00b |
8.32±0.05 |
350.51±3.57 |
1.00 |
100 |
| 1st ATPSc |
Top |
4,582.91±45.72 |
3.31±0.07 |
1,385.73±16.49 |
3.94±0.03 |
157.13±1.57 |
| 2nd ATPSd | 10 | 10,965.60±10.18 | 2.70±0.01 | 4,064.79±5.99 | 11.60±0.02 | 365.53±15.11 |
a crude enzyme extract from farmed giant catfish viscera (1:5 (w/v) of the minced viscera to 10mM Tris–HCl buffer with 10 mM CaCl2, pH 8.0).
b values are given as mean ± S.D. from triplicate determinations.
c 1st ATPS consists of 15% PEG2000-15%sodium citrate containing 70% (w/w) crude enzyme extract.
d 2nd ATPS consists of the top phase of the second cycle ATPS, additional 10% (w/w) sodium citrate.