Figure 8. Expression analysis by RT-PCR of LaLal2 and LaSCRL alleles in Leavenworthia SI and SC plants homozygous at the S locus.

(A) Expression analysis of LaLal2 alleles in stigmas collected 2 d before anthesis. Asterisks indicate bands corresponding to an alternatively spliced form of LaLal2 transcripts. The ACTIN gene was used as an internal control. (B) Expression analysis of LaSCRL alleles in anthers collected 2 d before anthesis. Because of the high sequence divergence between the different SCRL alleles, primer pairs used for amplification were allele-specific except for the a2 and a1-2 alleles, for which the same primer pair was used. The ACTIN gene was used as an internal control. Genomic DNA extracted from the four haplotypes was used to amplify SCRL with their respective primer pairs to show that all the primer pairs used in PCR reactions amplify SCRL.