Figure 3. Staining of 5hmC with a monoclonal antibody.

PN5 stage zygotes were collected directly from the oviduct and fixed and prepared for staining. They incubated with anti-5hmC or non-immune IgG for 1 h at room temperature (RT) or 18 h at 4°C after epitope retrieval by acid. Three representative images of each treatment are shown. These are representative of three independent replicates with more than 30 embryos per treatment in each replicate. The bar is 5 µm and all images are at the same magnification.