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. Author manuscript; available in PMC: 2013 May 14.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2010 Apr 29;30(7):1423–1429. doi: 10.1161/ATVBAHA.110.206680

Figure 1. Real-time PCR (RT-PCR) analyses of LacZ transgene mRNA in LacZK and LacZKNFI transgenic mice.

Figure 1

(A) Schematic of the wild-type and mutant VWF promoter sequence −487 to +247 fused to the LacZ gene. Transacting factors functioning as repressors (NFI, Oct-1, and NFY: ovals) and activators (Ets, NFY and GATA6: rectangles) are shown. TATA element: T (small square). Arrow at +1 represents transcription start site. Binding sequence of the repressors NFI and NFY in the wild-type (LacZK) and mutant promoters (LacZKNFI, LacZKNFY and LacZKNFI-NFY) are shown with base substitution mutations underlined and bolded. (B–E) RNA (1 μg) from various organs of (B) LacZK and (C–E) three independent lines of LacZKNFI transgenic mice (F2 generations) were analyzed by RT-PCR to detect LacZ transgene and endogenous GAPDH mRNA for normalization. Results represent the averages of mRNA from 2 littermates of each line for each organ.