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. 2013 May 14;8(5):e64251. doi: 10.1371/journal.pone.0064251

Table 1. Primers sequences used for quantitative real-time PCR assays.

Gene symbol Primer sequence
Accession Nb Forward Reverse
Cyclophilin A (Ppia) NM_017101 ATTCATGTGCCAGGGTGGT GATGCCAGGACCTGTATGCT
β2 microglobulin (β2M) NM_012512 ACCTGGGACCGAGACATGTA GAAGATGGTGTGCTCAATTGC
Hypoxanthin guanine phosphoribosyl transferase (HPRT) NM_012583 GTGTCATCAGCGAAAGTGGA ATGGCCACAGGACTAGAACG
Insulin receptor (INSR) NM_017071 AACAACCCTGGCAGCTGAACT TTCCAAGGTCTCTCTCGAA
Insulin Related Substrate 1 (IRS1) NM_0129969 ACAGAGAGTGGACCCCAATG ATGCTGCTACTGCTGCAAGA
Insulin Related Substrate 2 (IRS2) XM_0011076309 CTCTTTGCCCCGCTCTTAC GCTCCAGTCTCTCCTCTTCC
Insulin Like Growth Factor 2 (IGF2) NM_31511 CGCGGCTTCTACTTCAGCA GTCTCCAGGAGGGCCAAG
Insulin Like Growth Factor Receptor 1 (IGF1-R) NM_052807 TTGGAGATTTTGGTATGACACG GAGGGACTCGGGAGACATC
Insulin Like Growth Factor Receptor 2 (IGF2-R) NM_012756 CCGTGTGTGCTGTGGATAAG CACAGTCATCGCCATCAGAG
Glucose Transporter 1 (GLUT 1) NM_138827 ATTAAAAAGGTGACGGGCC CGGTGGTTCCATGTTTGATT
Glucose Transporter 3 (GLUT 3) RN_98055 GACCAAGCGACGGAGATC AGAGCTCCAGCACAGTGACC
Glucose Transporter 4 (GLUT 4) NP_036883 AGGCACCCTCACTACCCTTT ATAGCCCTTTTCCTTCCCAA
Amino acid transporter 1 (SNAT1) RN_162022 CGTGACTGACGCGTGCACACAC AGACGGGTGGCAGACAAACGC
Amino acid transporter 2 (SNAT2) NM_1801090 AAGAGCTGAAGAGCCGCAGC CGGCAAGCAAATACATCAGA
Amino acid transporter 4 (SNAT4) NM_001107078 CTGTTCCCCATCCGTACTTC GACGTTGTTGAGAGCGATGA
Amino acid transporter (LAT1) NM_031341 GATGCCTGCATCTGTCTCTTA CAATCAGCGCCAACACTTTA
Lipoprotein lipase 1 (LPL 1) NM_012598 AAGGTCATCTTCTGTGTCCA CAGCCCGACTTCTTCAGAGA
Lipoprotein lipase endothelial (LIPG) NM_001012741 GCAGGATCACTGGATTGGAT CAGCGTGTAGGTATGCAGGA
Lipoprotein lipase hormone sensitive (LIPE) NM_0128591 CTACTGGGATACAGCCTCGG AAGGCCATGTTGTCTTCTGC
Very low density lipoprotein receptor (VLDLR) NM_0131552 CCCTGAAGGAGTGTGCCATATC CAGCTGCACAGTCACATTCA
Placental Growth Factor (PLGF) RN_6960 CGCTAAAGACAGCCAACATC ATTCGCAGAGCACATCCTG
Vascular Endothelial Growth Factor A (VEGF) NM_031836 CTGGAGCGTTCACTGTGAG GCGAGTCTGTGTTTTTGCAG