Table 2. Symbiosis-associated genes identified in the EST analysis.
| Cluster ID | No. of clones | Annotation | SymM3 | SymM4B | SymM4 | ApoM3 | ApoM4B | ApoM4 | qRT-PCR* 1 |
| Rped-0008 | 62 | Cysteine-rich secreted protein | 1 | 0 | 61 | 0 | 0 | 0 | P<0.05 (M4) |
| Rped-0023 | 33 | Zinc carboxypeptidase | 0 | 32 | 1 | 0 | 0 | 0 | P<0.001 (M4) |
| Rped-0031 | 24 | gpi-anchor transamidase | 0 | 21 | 3 | 0 | 0 | 0 | N.S. |
| Rped-0043 | 19 | Cysteine-rich secreted protein | 0 | 2 | 17 | 0 | 0 | 0 | P<0.05 (M4) |
| Rped-0047 | 18 | CathepsinL | 0 | 0 | 18 | 0 | 0 | 0 | P<0.001 (M4) |
| Rped-0061 | 15 | Cysteine-rich secreted protein | 0 | 0 | 15 | 0 | 0 | 0 | N.S. |
| Rped-0077 | 12 | Cysteine-rich secreted protein | 0 | 0 | 12 | 0 | 0 | 0 | N.S. |
| Rped-0090 | 11 | Unknown secreted protein | 0 | 0 | 11 | 0 | 0 | 0 | P<0.001 (M4) |
| Rped-0095 | 10 | Cysteine-rich secreted protein | 0 | 0 | 10 | 0 | 0 | 0 | N.S. |
| Rped-0100 | 10 | Glyoxal oxidase | 10 | 0 | 0 | 0 | 0 | 0 | P<0.05 (M1,M2,M3) |
| Rped-0106 | 10 | Cysteine-rich secreted protein | 0 | 0 | 10 | 0 | 0 | 0 | P<0.05 (M4) |
*
1: Statistically significant differences between aposymbiotic and symbiotic insects in each midgut region by quantitative RT-PCR (t test; n = 8, see Figure 3B). N.S., not significant.