Table 3. Aposymbiosis-associated genes identified in the EST analysis.
| Cluster ID | No. of clones | Annotation | SymM3 | SymM4B | SymM4 | ApoM3 | ApoM4B | ApoM4 | qRT-PCR* 1 |
| Rped-0025 | 32 | c-type lysozyme | 0 | 0 | 0 | 5 | 26 | 1 | P<0.05 (M1,M2,M3,M4) |
| Rped-0033 | 23 | Defensin-like protein | 0 | 0 | 0 | 11 | 9 | 3 | P<0.05 (M1,M2,M3,M4) |
| Rped-0049 | 18 | Cathepsin B | 0 | 0 | 0 | 18 | 0 | 0 | N.S. |
| Rped-0053 | 17 | Unknown secreted protein | 0 | 0 | 0 | 0 | 17 | 0 | P<0.05 (M4) |
| Rped-0058 | 16 | Unknown protein | 0 | 0 | 0 | 0 | 14 | 2 | N.S. |
| Rped-0064 | 15 | Unknown protein | 0 | 0 | 0 | 6 | 7 | 2 | N.S. |
| Rped-0070 | 14 | Unknown secreted protein | 0 | 0 | 0 | 0 | 8 | 6 | P<0.001 (M4) |
*
1: Statistically significant differences between aposymbiotic and symbiotic insects in each midgut region by quantitative RT-PCR (t test; n = 8, see Figure 3B). N.S., not significant.