Table 1. Strains and plasmids used in this study.

Strains	Features	Source of reference
Escherichia coli
DH5α	F-Φ80lacZΔM15Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rk -, mk +) phoA supE44 thi-1 gyrA96 relA1 λ-	Laboratory collection
EC100D pir-116	F-mcrAΔ(mrr-hsdRMS-mcrBC)Φ 80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG pir-116(DHFR)	Laboratory collection
EC100D pir-116/R995+SPI-6	Strain with T6SSSPI-6 from S. Typhimurium cloned in plasmid R995	This study
EC100D pir-116/R995+SPI-19	Strain with T6SSSPI-19 from S. Gallinarum cloned in plasmid R995	[42]
DH5α/R995	Strain harboring an empty R995 vector	This study
DH5α/R995-VC6	Strain containing a derivative of plasmid R995 with a 1,209 bp DNAfragment of T6SSSPI-6 cloned from S. Typhimurium	This study
Salmonella Typhimurium
14028s	Wild-type strain	Laboratory collection
MTM753	14028s ΔphoN	This study
MTM35	14028s ΔSPI-6 T6SS	This study
MTM2640	14028s ΔclpV	This study
WT/R995	14028s containing an empty R995 vector	This study
MTM35R	MTM35 harboring R995 plasmid	This study
MTM35R6	MTM35 complemented with plasmid R995+SPI-6	This study
MTM35R19	MTM35 complemented with plasmid R995+SPI-19	This study
Plasmids
pKD46	bla PBAD bet exo pSC101 oriTs, AmpR	[43]
pEKA30	IncQ plasmid that constitutively express Cre recombinase, AmpR	[44]
pCLF2	Red-swap redesigned vector, CamR	[50]
pCLF4	Red-swap redesigned vector, KanR	[50]
pVEX1212	Suicide vector harboring a loxP site followed by a SpR cassette	[44]
pVEX2212	Suicide vector harboring a loxP site followed by a CamR cassette	[44]
R995	Self-transmissible broad-host range IncP vector	[44]
R995-VC6	A derivative of plasmid R995 with a 1,209 bp DNA fragment of T6SSSPI-6cloned from S. Typhimurium	This study
R995+SPI-6	T6SSSPI-6 cluster from S. Typhimurium 14028s cloned in vector R995	This study
R995+SPI-19	T6SSSPI-19 cluster from S. Gallinarum 287/91 cloned in vector R995	[42]