Table 1. Effects of δ/ω-PLTX-Pt1a (20 nM) on the voltage-dependent Na+ currents.
Vth (mV)a | Vh (mV)b | Ip (nA)c | Iss (nA)c | Ip/Iss | ♦ (ms)d | r (%)e | |
Before | −38.6±9.0 | −45.5±7.6 | −1.43±0.64 | −0.15±0.12 | 25.9±25.1 | 1.84±0.34 | 0.76±0.06 |
After | −45.7±9.7* | −50.0±5.4 | −1.18±0.66 | −0.22±0.15 | 9.2±9.4 | 3.44±1.58** | 0.79±0.08 |
n = 7 | n = 4 | n = 7 | n = 7 | n = 6 | n = 14 | n = 4 |
Results are shown as Mean±SD. * p<0.01. **p<0.002. (Paired student's t-test).
a. Vth is the threshold membrane potential at which the amplitude of inward Na+ current is larger than 50 pA which can be confidently distinguished from the noise level;
b. Vh is the membrane potential of half-inactivation;
c. Ip and Iss are the amplitudes of peak and steady-state Na+ currents respectively;
d. ♦is the decay rate of the inactivating Na+ current evoked by depolarizing pulse to −20 mV from the holding potential of −80 mV. The value was obtained by single exponential fitting of the current decay phase. In 2 of the 14 cells, currents were better fitted with a double-exponential function, of which ♦1 was shown here.
e. r is the fractional recovery from inactivation of Na+ currents at −10 mV, assessed by the double pulse paradigm with an interpulse interval of 500 ms.