Figure 4. The 4q32A>C mutation leads to reduced enhancer activity and POU2F1 and YY1 binding.

(A) Luciferase assays of the enhancer activity using the thyroid cancer cell line BCPAP. **, p-value<0.005. (B) ChIP assay for transcription factors POU2F1 and YY1 using antibodies against POU2F1 and YY1 in lymphoblastoid cell lines. The control and patient cell lines are the same as in Figure 2. **, p-value<0.01. Error bars represent ±SD of at least three independent experiments.