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. 2013 May 14;8(5):e64416. doi: 10.1371/journal.pone.0064416

Figure 3. TLR2-mediated immune responses induced by recombinant SpaCBA-piliated lactococcal cells.

Figure 3

Stimulation of TLR2-dependent NF-κB activation in the HEK-TLR2 cell line by normalized cultures of recombinant SpaCBA-piliated lactococcal cells (GRS1185, GRS1195, GRS1211, and GRS1223) was carried out according to the method described in Materials and Methods. GRS71 and GRS1052 lactococcal cells as well as DMEM cell culture media were included as negative controls. TLR2-agonist lipopeptide Pam3CSK4 (1 ng/ml) was used a positive control. Measurements were done in quadruplicate and the experiment was performed three times. Error bars are standard error of mean (SEM). Differences between data (GRS1185, GRS1195, GRS1211, or GRS1223) from pairwise comparisons against GRS71 data are considered extremely significant (P<0.001).