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. 2013 May 14;8(5):e63286. doi: 10.1371/journal.pone.0063286

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© 2013 Krylova et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A, Amino acids inserted (black font, SV40 NLS underlined) between two mCherry FPs (orange font) within the mCherry_NLSmCherry nuclear marker. The insertion sequence and location is similar for the other FP_NLSFP nuclear markers created in this study. B, Nuclear fluorescence of the mCherry_NLSmCherry marker stably expressed in a HeLa cell line in relationship to nuclei stained with Hoechst 33342. The locations of a YFP-tagged Androgen Receptor (AR) co-expressed in this cell line also are shown. The cells were grown in media containing testosterone, which translocates the AR into the cell nuclei. Images were captured with a 10x objective. *, mitotic cell. #, dying cell.